Alcohol showed that it cannot break down the membrane as much as e. The used pasteurs and cuvettes go in the pots on the bench. A prepared beetroot cylinder is placed in each, and these are left to sit for 45 minutes with occasional agitation. Disposal of Material and Equipment All gloves and pipette sleeves must be disposed of in the blue waste bins.
However when we increase the temperature the relatively weak forces holding the different parts of the polypeptide chains together like hydrogen bonds can be disrupted very easily- this damages the vacuole and makes holes in the cell membrane, inducing leakage.
General Aspects Gloves must be worn at all times to protect from pigment staining. The bottles were left to incubate for 20 minutes and then the beetroot cylinders were removed. Additionally, the highest concentration of each solvent showed the most damage.
The pieces cut had roughly the same surface area — but not exactly the same. I can firmly conclude that there are no apparent anomalies in my results and none of my sources of error or limitations of my experiment are enough to deem my results unreliable. Lab coats must be fully fastened to protect clothes from staining.
To avoid the random error, the implement for cutting the red cabbage into discs should have a smaller diameter, or the diameter of the test tubes should be greater in order to examine the sinking of the leaf discs.
Results are then recorded and runt through DataAnalysis Effect of freezing on membrane permeability Beetroot cylinders are frozen in liquid nitrogen The beetroot cylinders are then placed in 15cm3 of distilled water at room temperature.
Following incubation, damage to cell membrane integrity was assessed by quantifying leakage of the pigment betacyanin via measuring light absorbance at nm. Bottles containing solvents must never be emptied.
The function and permeability of the cell membrane depends on its whole structure. It would also have been better to have had the time to repeat each temperature more times to make the results more reliable and so I could be sure the results were not gained through chance.
Beetroot contains a red pigment called betacyanin, which is located in the large central vacuole of the beetroot cells. All of the other items go into separate white buckets.
The more pigment releases, the more the membrane is broken down. Spectrophotometer readings are taken as above, at nm, calibrated by the control solution, for each tube. For temperature being the independent variable, test tube 2 was the contol test, beacuse it showed what happens the disitlled water will not change colour at room temperature, beacuse the hydrophilic heads face the water, and the hydrophobic tails face each other.
An Introduction for the Life and Medical Sciences. This would have had a slight effect on my results because the rate of diffusion of betacyanin particles across the plasma membrane is increased, as the surface area of the beetroot increases.
Secondly, when I washed each of the beetroot pieces I may not have removed all of the red pigment on the outside, so this would have affected my results very slightly. Get Access Membrane Lab Report Essay Sample The cell membrane consists of mostly phospholipids and proteins which gives the cell its selectively permeable nature.
Method s Effect of Temperature on Beet Membrane Permeability Beetroot cylinders were cut into 2x 1cm strips, then rinsed for 10 minutes under running water to remove pigmentation from damaged cells 7 boiling tubes are prepared by placing 15 cm3 of distilled water in each tube One beetroot cylinder is transferred into a beaker in a 70 Celsius waterbath for one minute, and then transferred again into a tube of 15cm3 distilled water.
An increase in temperature will affect the permeability of the cell membrane. The aim of this experiment is to observe the effect of physical and chemical stress on the membrane permeability of beetroot. Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes.
Beetroot contains a red pigment called betacyanin, which is located in the large central vacuole of the beetroot cells. * My beetroot discs are 1mm in length because the larger the surface area, the more effect would be there for the diffusion rate on the membrane permeability of beetroot.
* Place the beetroot discs in a beaker of cold, running water (at least 2 min). Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes. Beetroot contains a red pigment called betacyanin, which is located in the large central vacuole of the beetroot cells.
Published: Mon, 5 Dec Abstract. The effect of various temperatures on the membrane permeability of Beta Vulgaris, more commonly known as the red beet, will be investigated in this experiment. Effect of Temperature on Membrane Permeability Beetroot. Print Reference this. Disclaimer: If you are the original writer of this essay and no longer wish to have the essay published on the UK Essays website then please click.
Permeability of Beetroot Cell Membranes An experiment to investigate how temperature affects the Permeability of beetroot cell membranes Interpretation of Results.The permability of membranes in beetroot essay