In my experiment the substrate was the hydrogen peroxide, the enzyme that we used was hydrogen peroxide and the product that was formed was oxygen and water.
Now repeat the experiment 20 times for each pH in order to establish enough data so that an average value can be taken and so a more accurate graph can be produced.
Any spillages will be mopped up and any broken glass reported and cleared up carefully and disposed in a special glass bin. There are two theories that show how enzymes work and are shown below. However all the aspects mentioned above could have affected the results but a general trend was discovered therefore showing that they really did not have a huge affect.
This graph shows that the enzyme activity reacts best at 40. The rate of reaction was also found by dividing 1 by the average time taken and then multiplied it by to make it easier to plot on a graph. The manipulated variable in this experiment is the pH where buffer solution is used to alter it.
The anomaly could have been obtained due to many reasons.
There are several factors that could affect our rate of reaction. One check, previously mentioned, was the measurement of a data set using a colorimeter. Evaluation The experiment can be judged a success as a general trend was discovered.
Enzymes are very sensitive to pH changes and they all have an optimum pH where they work best at. They speed up chemical reactions. I can tell that the catalase in my experiment is a suitable enzyme to break down the H as it will form oxygen as a product which is unharmful Denaturing Denaturing is the damage to the protein structure of an enzyme.
However all the aspects mentioned above could have affected the results but a general trend was discovered therefore showing that they really did not have a huge affect.
There are a couple of possible explanations for this apparent anomaly. Also, it is not difficult to imagine setting up equipment that would work along the lines of the colorimeter, measuring light levels through a sample, only set to stop a timer the moment a particular uniform reading was reached.
This conclusion was reached using scientific knowledge and research. The duodenum produces sodium hydrogencarbonate that provides the alkaline conditions for the trypsin to work in. This is important because if one sample of potato is allowed to soak in a solution for more time it may become more acidic or more alkali that it should and therefore taint results.
As the trypsin reacted during the experiment, the marker pen cross would have appeared gradually as the milk sample cleared. C Surface Area — Reactions can react faster when solids are cut into smaller pieces. It must be pointed out at this time that a shorter time recording indicates an increase in trypsin activity, so the table and graph are ones of inverse correlation; as the graph or data go down, the value enzyme activity goes up.
The beta-pleated sheets are composed of chains which are side by side and are connected by Hydrogen bonds.
The pH is very alkaline in the duodenum as it has to neutralise the acidic hydrochloric acid that is made in the stomach. An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions.
Investigation on the effect of pH on enzymes Essay Sample.
Design Experiment. How does pH of potatoes affect the breakdown of starch by the enzyme Salivary Amylase? Effect of Ph Level and Hydrogen Peroxide on Catalase.
Print Reference this. Enzymes will be denatured, if the optimum ph and substrate concentrations at which they work best are altered and this will result in inactivity of the reaction (www3).
The two independent variables in the investigation were ph and hydrogen peroxide. Each. Essay about Amylase Investigation; Essay about Amylase Investigation I am going to investigate the effect of pH and temperature on the activity of the enzyme amylase.
this lab our group observed the role of pancreatic amylase in the digestion of starch and the optimum temperature and pH that affects this enzyme. Enzymes are located. An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.
Investigation on the effect of pH on enzymes Essay Sample. Design Experiment. How does pH of potatoes affect the breakdown of starch by the enzyme Salivary Amylase?Investigation on the effect of ph on enzymes essay