For eye contact, immediately flush eyes with plenty of water. Therefore, there were lots of possible errors which could have been made during this experiment.
Our previous studies have shown that the expression of antioxidant enzymes was altered in copper-deficient rat liver.
Size of test tube Different sized test tubes will hold different volumes of O2 and will give incorrect measurements with the ruler Keep all the test tubes the same size — with volume of 30cm3 Uncontrolled Variables The variables that will be difficult to control include air pressure in the lab and the volume of the liver.
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Livers were analyzed for copper content. As seen in figure 14, the effectiveness of an enzyme is the greatest at its optimum pH and slowly degenerates as the surrounding pH shifts either lower or higher. Posted April 25,Part 2 For this experiment, several different outcomes were recorded for the catalyzed decomposition of starch into simpler monosaccharides via amylase.
What does he bring us. The six factors are: Based on these data, how does substrate concentration affect enzyme action.
Dependent Variables The dependent variable is the height of the reaction, and the bubbles of gas produced will be measured in millimetres mm.
This means the experiment was quite precise since it had low probability of happening due to chance. Mark the level with a Based on these data, how does temperature affect enzyme action.
At higher temperature, the vibration is produced inside enzymes due to heat. Wait until the temperature has reached 37. Hepatic mitochondria and microsomes humans only were isolated, and lipid peroxidation was measured by lipid-conjugated dienes and thiobarbituric acid-reacting substances.
C then use it instead. We examined effects of copper deficiency on metabolism in major structures of the auditory system. Lipid peroxidation of polyunsaturated fatty acids exposed to oxygen leads to rancidity in foods. The elimination of hydrogen peroxide by glutathione can be written as the reaction: Oxidative damage to DNA is ten times greater in rats than in humans.
There were lots of errors and possible errors which were made during the experiment.
This test tube was placed in 90 degrees hot water for five minutes, and the colour of the liquid beef liver has changed from a dark pink colour to a brownish colour as shown in the picture.
At pH 3 more than times more acidic than its functioning pH range, the amylase denatured and was no longer able to bind with the substrate at its active site to catalyze the reaction.
The hydrogen peroxide must be quickly degraded or converted, and catalase accomplishes this task because one molecule of catalase can deal with six million molecules of hydrogen peroxide in one minute.
There would be blue pcell dots everywhere. This shows plastic deformation due to its ductile behavior The Line weaver-Burk plot is shown in Fig. There was indefinite amount of substrate concentration which could have definitely caused an error.
Water which was at the room temperature released the most amount of oxygen because it had highest displacement of water.
Published: Mon, 5 Dec Enzymes are catalysts made within the human body. Catalysts naturally, lower the activation energy required for reactions.
The lower the activation energy is, the faster the rate of reaction is, and therefore enzymes speed up reactions in. - Investigating Enzyme Activity Aim: To investigate how the concentration of hydrogen peroxide effects the rate of reaction of an enzyme (catalase) Variables: These factors could effect the rate of reaction on an enzyme: · pH · Concentration · Temperature · Surface Area pH -.
Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate, and also Read more Discover the affects of PH on the enzyme catalase.
Composition of the culture medium, growth phase, and temperature play important roles in the sensitivity of Escherichia coli to H2O2. The medium and growth phase affected the sensitivity of the cells to H2O2 by modifying the amount of catalase synthesized by them, whereas the effect of temperature was due to the thermolability of the enzyme.
Factors affecting the rate of catalase activity The extent at which environmental factors affect the rate of catalase activity was discovered in this lab. The assay system, in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20mL of hydrogen peroxide, was used to test several enzyme.
Factors Affecting the Rate of Enzyme activity Essay examples. Length: words ( double-spaced pages) The Effect of Temperature on the Rate of Enzyme Activity Essay examples - The first way is by helping the substrates to bind together.
- The Effect of Substrate Concentration on the Rate of Activity of the Enzyme Catalase.Factors affecting the rate of catalase activity essay